copt dap solution  (Thermo Fisher)

Journal: Therapeutic Advances in Infectious Disease

Article Title: A prospective study to evaluate high dose daptomycin pharmacokinetics and pharmacodynamics in Staphylococcus spp. infective endocarditis

doi: 10.1177/20499361241296232

Figure Lengend Snippet: Pharmacokinetics of daptomycin (data expressed as median (IQR)).

Article Snippet: The first one (QcH) was prepared by diluting 80 μL of the second daptomycin stock solution (5 mg/mL) with 4920 μL of plasma in a 10-mL Eppendorf tube.

Techniques: Drug discovery

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Failures in Clinical Treatment of Staphylococcus aureus Infection with Daptomycin Are Associated with Alterations in Surface Charge, Membrane Phospholipid Asymmetry, and Drug Binding

doi: 10.1128/aac.00719-07

Figure Lengend Snippet: FIG. 1. Population analyses of study strains upon exposure to a range of vancomycin (A) or daptomycin (B) concentrations (conc.). These data represent the means ( SD) for two separate assays. See the text for details.

Article Snippet: Calcein-loaded S. aureus cells (final inoculum, 106 CFU/ml) were diluted into the sublethal concentrations of test AP or daptomycin solutions (i.e., 0.5 g/ml tPMP-1, 10 g/ml hNP-1, 32 g/ml gramicidin D, and 1.5 g/ml daptomycin) based on our pilot studies and incubated at 37°C for 120 min. At selected time points, calcein retention was quantified using a Turner digital fluorometer (model 450; Barnstead/Thermolyne Corp., Dubuque, IA) (49).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Failures in Clinical Treatment of Staphylococcus aureus Infection with Daptomycin Are Associated with Alterations in Surface Charge, Membrane Phospholipid Asymmetry, and Drug Binding

doi: 10.1128/aac.00719-07

Figure Lengend Snippet: FIG. 2. Membrane depolarization by daptomycin, RP-1, and Triton X-100 by flow cytometry at 60-min exposures. These data represent the means for three separate assays. See the text for details.

Article Snippet: Calcein-loaded S. aureus cells (final inoculum, 106 CFU/ml) were diluted into the sublethal concentrations of test AP or daptomycin solutions (i.e., 0.5 g/ml tPMP-1, 10 g/ml hNP-1, 32 g/ml gramicidin D, and 1.5 g/ml daptomycin) based on our pilot studies and incubated at 37°C for 120 min. At selected time points, calcein retention was quantified using a Turner digital fluorometer (model 450; Barnstead/Thermolyne Corp., Dubuque, IA) (49).

Techniques: Membrane, Cytometry

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Failures in Clinical Treatment of Staphylococcus aureus Infection with Daptomycin Are Associated with Alterations in Surface Charge, Membrane Phospholipid Asymmetry, and Drug Binding

doi: 10.1128/aac.00719-07

Figure Lengend Snippet: FIG. 3. Membrane permeabilization of the study strains by sublethal concentrations of two study antimicrobial peptides (tPMP-1 [A] and hNP-1 [B]) and daptomycin (C) by using the calcein release assay. These data represent the means ( SD) for three separate assays. See the text for details.

Article Snippet: Calcein-loaded S. aureus cells (final inoculum, 106 CFU/ml) were diluted into the sublethal concentrations of test AP or daptomycin solutions (i.e., 0.5 g/ml tPMP-1, 10 g/ml hNP-1, 32 g/ml gramicidin D, and 1.5 g/ml daptomycin) based on our pilot studies and incubated at 37°C for 120 min. At selected time points, calcein retention was quantified using a Turner digital fluorometer (model 450; Barnstead/Thermolyne Corp., Dubuque, IA) (49).

Techniques: Membrane, Release Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Failures in Clinical Treatment of Staphylococcus aureus Infection with Daptomycin Are Associated with Alterations in Surface Charge, Membrane Phospholipid Asymmetry, and Drug Binding

doi: 10.1128/aac.00719-07

Figure Lengend Snippet: FIG. 4. Autolysis of the study strains, as determined by the change in OD600 over time for Triton X-100 (A), daptomycin (B), and vancomycin (C). Data represent the means for at least two separate runs. Data are depicted as the percent change from the time zero (T0 [preexposure]) value, set at 100%. See the text for details.

Article Snippet: Calcein-loaded S. aureus cells (final inoculum, 106 CFU/ml) were diluted into the sublethal concentrations of test AP or daptomycin solutions (i.e., 0.5 g/ml tPMP-1, 10 g/ml hNP-1, 32 g/ml gramicidin D, and 1.5 g/ml daptomycin) based on our pilot studies and incubated at 37°C for 120 min. At selected time points, calcein retention was quantified using a Turner digital fluorometer (model 450; Barnstead/Thermolyne Corp., Dubuque, IA) (49).

Techniques: